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Toxoplasma Movies  

trails



Circular gliding of tachyzoites on serum-coated glass (TgMovie1.mov) (Sebastion Håkansson)

Helical gliding of tachyzoites on serum-coated glass (TgMovie2)  (Sebastian Håkansson)

Tachyzoite invasion of macrophages (TgMovie3) (Hiroshi Morisaki)

Hyper kinetic behavior of tachyzoites treated with jasplakinolide (TgMovie4) (Sebastian Håkansson)

Calcium transients during tachyzoite gliding motility (TgMovie5) (Jennie Lovett)

Calcium quenching during cell invasion by tachyzoite (TgMovie6) (Jennie Lovett)

Passage of both raft and nonraft lipids through the moving junction in BHK cells costained with FAST DiOC18 (green) and DiIC16 (red).  Time-lapse images at a single focal plane were collected at 1 sec intervals.  (TgMovie7) (Audra Charron)

Passage of a nonraft cytosolic leaflet protein through the moving junction during invasion of T. gondii parasites into BHK transfectants expressing S15-GFP.  Time-lapse images were captured at 1 sec intervals by wide-field video microscopy.  (TgMovie8) (Audra Charron)

Passage of a raft cytosolic leaflet protein through the moving junction during invasion of T. gondii parasites into BHK transfectants expressing L10-GFP.  Time-lapse images were captured at 1 sec intervals by wide-field video microscopy.  (TgMovie9) (Audra Charron)

Accessibility of a multipass transmembrane raft protein to the PVM during invasion by T. gondii parasites into BHK transfectants expressing CCR5-GFP.  Time-lapse images were captured at 1 sec intervals by wide-field video microscopy.  (TgMovie10) (Audra Charron)

 

Malaria Movies

 

Circular gliding of P. berghei sporozoites (MalariaMovie1) (Courtesy of Jim Dvorak, NIH)

Helical gliding of P. berghei sporozoites (MalariaMovie2) (Courtesy of Jerome Vanderberg, NYU)

Cell invasion by P. berghei sporozoites (MalariaMovie3) (Courtesy of Jerome Vanderberg, NYU)

Gliding of ookinetes on mosquito midgut (MalariaMovie4) (Courtesy of Jim Dvorak, NIH)

 

Cryptosporidium Movies

 

Multiple sporozoites of C. parvum gliding on poly-lysine coated glass (CpMovie1) (Dawn Wetzel)

Close-up of circular gliding by C. parvum sporozoites (CpMovie2) (Dawn Wetzel)

Close-up of helical gliding by C. parvum sporozoites (CpMovie3) (Dawn Wetzel)

 

Comparative gliding of Cryptospordium and Toxoplasma Sporozoites



Wetzel DM, et al. Gliding motility leads to active cellular invasion by Cryptosporidum Sporozoites. Infect. Immun. in press (Link to movies).



Gregarine Movies

 

Gregrina polymorpha gliding on substrate (scale bar = 100 nm) (GregMovie1) (Courtesy of Conrad King, London)

Translocation of beads along the surface of G. polymorpha (posterior end at top) (GregMovie2) (Courtesy of Conrad King, London)

For more information on Gregarines.




For a more complete description of the videos above, please see the  materials that were published previously as a supplement for the following article.(pdf)

Sibley LD: Intracellular Parasite Invasion Strategies.  Science, 2004, 304, 248-253. PMID: 15073368

 

 scope
Time-lapse images of Toxoplasma and
Cryptosporidiam were recorded on the microscope
shown above.